anti plexinb2  (R&D Systems)

Journal: PeerJ

Article Title: Semaphorin4A promotes lung cancer by activation of NF-κB pathway mediated by PlexinB1

doi: 10.7717/peerj.16292

Figure Lengend Snippet: After stimulation by Sema4A-Fc protein, PlexinB1, PlexinB2, PlexinD1 blocking antibodies, and IgG antibody alone or in combination, the proliferative and migratory abilities of LC cells were assessed by colony formation (A) and scratch (B) assays, respectively. Magnification for (B): ×40. @ P < 0.05, and @@ P <0.01 vs. Control; # P < 0.05, and ## P < 0.01 vs. IgG; − P < 0.05, and −− P < 0.01 vs. Sema4A-Fc+IgG. Results were presented as mean ± SD. n = 3. Note: Sema4A: semaphorin 4A; LC, lung cancer.

Article Snippet: The PlexinB1, PlexinB2, PlexinD1 blocking antibodies and IgG antibody were supplied by R&D Systems (Minneapolis, MN, USA).

Techniques: Blocking Assay, Control

Journal: PeerJ

Article Title: Semaphorin4A promotes lung cancer by activation of NF-κB pathway mediated by PlexinB1

doi: 10.7717/peerj.16292

Figure Lengend Snippet: After stimulation by Sema4A-Fc protein, PlexinB1, PlexinB2, PlexinD1 blocking antibodies, and IgG antibody, alone or in combination, the invasive ability and the viability of LC cells were assessed by Transwell (A) and MTT (B) assays, respectively. Magnification for (B): ×200. @ P <0.05, and @@ P < 0.01 vs. Control; # P < 0.05, and ## P <0.01 vs. IgG; − P < 0.05, and −− P < 0.01 vs. Sema4A-Fc+IgG. Results were presented as mean ± SD. n = 3. Note: Sema4A: semaphorin 4A; LC, lung cancer; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: The PlexinB1, PlexinB2, PlexinD1 blocking antibodies and IgG antibody were supplied by R&D Systems (Minneapolis, MN, USA).

Techniques: Blocking Assay, Control